DTU Chemistry - Pippi


DTU Chemistry

Isoform-Specific Substrate Inhibition Mechanism of Human Tryptophan Hydroxylase.
Tidemand KD, Peters GH, Harris P, Stensgaard E, Christensen HEM.
 2017 Nov 21;56(46):6155-6164.

The influence of different linker modifications on the catalytic activity and cellulose affinity of cellobiohydrolase Cel7A from Hypocrea jecorina.
Badino SF, Bathke JK, Sørensen TH, Windahl MS, Jensen K, Peters GHJ, Borch K, Westh P.
Protein Eng Des Sel. 2017 Jul 1;30(7):495-501.

Small-Angle X-ray Scattering Data in Combination with RosettaDock Improves the Docking Energy Landscape.
Sønderby P, Rinnan Å, Madsen JJ, Harris P, Bukrinski JT, Peters GHJ.
J Chem Inf Model. 2017 Oct 23;57(10):2463-2475.

Glucagon-like Peptide 1 Conjugated to Recombinant Human Serum Albumin Variants with Modified Neonatal Fc Receptor Binding Properties. Impact on Molecular Structure and Half-Life.
Bukrinski JT, Sønderby P, Antunes F, Andersen B, Schmidt EGW, Peters GHJ, Harris P.
 2017 Sep 12;56(36):4860-4870.

Insulin fibrillation: The influence and coordination of Zn2.
Frankær CG, Sønderby P, Bang MB, Mateiu RV, Groenning M, Bukrinski J, Harris P.
J Struct Biol. 2017 Jul;199(1):27-38.

Soluble 1:1 complexes and insoluble 3:2 complexes - Understanding the phase-solubility diagram of hydrocortisone and γ-cyclodextrin.
Schönbeck C, Madsen TL, Peters GH, Holm R, Loftsson T.
Int J Pharm. 2017 Oct 15;531(2):504-511.

The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.

Skjoldager N, Blanner Bang M, Rykær M, Björnberg O, Davies MJ, Svensson B, Harris P, Hägglund P.
Sci Rep.
 2017 Apr 11;7:46282.

Revealing the Compact Structure of Lactic Acid Bacterial Heteroexopolysaccharides by SAXS and DLS.

Khan S, Birch J, Harris P, Van Calsteren MR, Ipsen R, Peters GH, Svensson B, Almdal K.
 2017 Mar 13;18(3):747-756.


Interaction mechanisms between polycyclic aromatic hydrocarbons (PAHs) and organic soil washing agents.
Greish S, Rinnan Å, Marcussen H, Holm PE, Christensen JH.
Environ Sci Pollut Res Int.
 2017 Oct 15.

Tracking hydrophobicity state, aggregation behaviour and structural modifications of pork proteins under the influence of assorted heat treatments.
Mitra B, Rinnan Å, Ruiz-Carrascal J.
Food Res Int.
 2017 Nov;101:266-273.

Predicting the ethanol potential of wheat straw using near-infrared spectroscopy and chemometrics: The challenge of inherently intercorrelated response functions.

Rinnan Å, Bruun S, Lindedam J, Decker SR, Turner GB, Felby C, Engelsen SB.
Anal Chim Acta.
 2017 Apr 15;962:15-23. 

Ludwig-Maximilans University Munich

Notorious but not understood: How liquid-air interfacial stress triggers protein aggregation.
Koepf EEisele SSchroeder RBrezesinski GFriess W.

Int J Pharm. 2017 Dec 26;537(1-2):202-212

Stress-responsive FKBP51 regulates AKT2-AS160 signaling and metabolic function.
Balsevich G, Häusl AS, Meyer CW, Karamihalev S, Feng X, Pöhlmann ML, Dournes C, Uribe-Marino A, Santarelli S, Labermaier C, Hafner K, Mao T, Breitsamer M, Theodoropoulou M, Namendorf C, Uhr M, Paez-Pereda M, Winter G, Hausch F, Chen A, Tschöp MH, Rein T, Gassen NC, Schmidt MV.
Nat Commun.
 2017 Nov 23;8(1):1725.

A comparison of nanoparticullate CpG immunotherapy with and without allergens in spontaneously equine asthma-affected horses, an animal model. Klier J, Geis S, Steuer J, Geh K, Reese S, Fuchs S, Mueller RS, Winter G, Gehlen H.
Immun Inflamm Dis.
 2017 Nov 1.

Formation of mannitol core microparticles for sustained release with lipid coating in a mini fluid bed system.

Wang B, Friess W.
Eur J Pharm Biopharm.
 2017 Nov;120:126-132.

Spray drying of silica microparticles for sustained release application with a new sol-gel precursor.
Wang B, Friess W.
Int J Pharm.
 2017 Oct 30;532(1):281-288.

Scale-up of water-based spider silk film casting using a film applicator.
Agostini E, Winter G, Engert J. 
Int J Pharm.
 2017 Oct 30;532(1):13-20. 

CONTAMINATION OF ANTI-VEGF DRUGS FOR INTRAVITREAL INJECTION: How Do Repackaging and Newly Developed Syringes Affect the Amount of Silicone Oil Droplets and Protein Aggregates?

Schargus M, Werner BP, Geerling G, Winter G.
 2017 Aug 21. 

The film tells the story: Physical-chemical characteristics of IgG at the liquid-air interface.

Koepf E, Schroeder R, Brezesinski G, Friess W.
Eur J Pharm Biopharm. 2017 Oct;119:396-407. 

Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models.
Strüver K, Friess W, Hedtrich S.
Skin Pharmacol Physiol.

Impact of plasma protein binding on cargo release by thermosensitive liposomes probed by fluorescence correlation spectroscopy.
Mittag JJ, Kneidl B, Preiβ T, Hossann M, Winter G, Wuttke S, Engelke H, Rädler JO.
Eur J Pharm Biopharm.
 2017 Oct;119:215-223. 

Thermo-Optical Protein Characterization for Straightforward Preformulation Development.
Wanner R, Breitsprecher D, Duhr S, Baaske P, Winter G.
J Pharm Sci. 2017 Oct;106(10):2955-2958.

Lyophilization Cycle Design for Dual Chamber Cartridges and a Method for Online Process Control: The "DCC LyoMate" Procedure.

Korpus C, Friess W.
J Pharm Sci. 2017 Aug;106(8):2077-2087.

Long-term release and stability of pharmaceutical proteins delivered from solid lipid implants.
Vollrath M, Engert J, Winter G.
Eur J Pharm Biopharm. 2017 Aug;117:244-255. 

Lyophilized Drug Product Cake Appearance: What Is Acceptable?

Patel SM, Nail SL, Pikal MJ, Geidobler R, Winter G, Hawe A, Davagnino J, Rambhatla Gupta S.
J Pharm Sci.
 2017 Jul;106(7):1706-1721.

Trends on Analytical Characterization of Polysorbates and Their Degradation Products in Biopharmaceutical Formulations.
Martos A, Koch W, Jiskoot W, Wuchner K, Winter G, Friess W, Hawe A.
J Pharm Sci.
 2017 Jul;106(7):1722-1735.

cmRNA/lipoplex encapsulation in PLGA microspheres enables transfection via calcium phosphate cement (CPC)/PLGA composites.
Utzinger M, Jarzebinska A, Haag N, Schweizer M, Winter G, Dohmen C, Rudolph C, Plank C.
J Control Release. 2017 Mar 10;249:143-149. 

Twin-screw extruded lipid implants containing TRP2 peptide for tumour therapy.
Even MP, Bobbala S, Gibson B, Hook S, Winter G, Engert J.
Eur J Pharm Biopharm. 2017 May;114:79-87.

Evaluation of Heat Flux Measurement as a New Process Analytical Technology Monitoring Tool in Freeze Drying.
Vollrath I, Pauli V, Friess W, Freitag A, Hawe A, Winter G.
J Pharm Sci.
 2017 May;106(5):1249-1257.

Needle-Free Injection of Vesicular Phospholipid Gels-A Novel Approach to Overcome an Administration Hurdle for Semisolid Depot Systems.

Breitsamer M, Winter G.
J Pharm Sci.
 2017 Apr;106(4):968-972.

Evaluation of Different Holder Devices for Freeze-Drying in Dual-Chamber Cartridges With a Focus on Energy Transfer.

Korpus C, Friess W.
J Pharm Sci. 2017 Apr;106(4):1092-1101. 

Preservative loss from silicone tubing during filling processes.

Saller V, Matilainen J, Rothkopf C, Serafin D, Bechtold-Peters K, Mahler HC, Friess W.
Eur J Pharm Biopharm. 2017 Mar;112:109-118.


Interfacial Adsorption of Monoclonal Antibody COE-3 at the Solid/Water Interface.
Pan F, Li Z, Leyshon T, Rouse D, Li R, Smith C, Campana M, Webster JRP, Bishop SM, Narwal R, van der Walle CF, Warwicker J, Lu JR. 
ACS Appl Mater Interfaces. 2017 Dec 7

Decomposing Dynamical Couplings in Mutated scFv Antibody Fragments into Stabilizing and Destabilizing Effects.
Ettayapuram Ramaprasad AS, Uddin S, Casas-Finet J, Jacobs DJ.
J Am Chem Soc. 2017 Dec 6;139(48):17508-17517. 

Controlling the bioactivity of a peptide hormone in vivo by reversible self-assembly.
Ouberai MM, Dos Santos ALG, Kinna S, Madalli S, Hornigold DC, Baker D, Naylor J, Sheldrake L, Corkill DJ, Hood J, Vicini P, Uddin S, Bishop S, Varley PG, Welland ME.
Nat Commun. 
2017 Oct 18;8(1):1026.

A simple supramolecular assay for drug detection in urine.
Sonzini S, McCune JA, Ravn P, Scherman OA, van der Walle CF. 
Chem Commun (Camb)
. 2017 Aug 3;53(63):8842-8845.

Neutron Reflection Study of Surface Adsorption of Fc, Fab, and the Whole mAb.

Li Z, Li R, Smith C, Pan F, Campana M, Webster JRP, van der Walle CF, Uddin S, Bishop SM, Narwal R, Warwicker J, Lu JR.
ACS Appl Mater Interfaces.
 2017 Jul 12;9(27):23202-23211.

Dipicolinic acid as a novel spore-inspired excipient for antibody formulation.

Batalha IL, Ke P, Tejeda-Montes E, Uddin S, van der Walle CF, Christie G.
Int J Pharm. 2017 Jun 30;526(1-2):332-338.

Antibody adsorption on the surface of water studied by neutron reflection.
Smith C, Li Z, Holman R, Pan F, Campbell RA, Campana M, Li P, Webster JR, Bishop S, Narwal R, Uddin S, van der Walle CF, Lu JR. MAbs. 2017 Apr;9(3):466-475. 

Lund University

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei G, Vazdar M, Jensen MR, Cragnell C, Mason PE, Heyda J, Skepö M, Jungwirth P, Lund M.
Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):11428-11433.

Assembling oppositely charged lock and key responsive colloids: A mesoscale analog of adaptive chemistry.
Mihut AM, Stenqvist B, Lund M, Schurtenberger P, Crassous JJ.
Sci Adv. 2017 Sep 15;3(9):e1700321. 

Steering patchy particles using multivalent electrolytes.
Abrikosov AI, Stenqvist B, Lund M.
Soft Matter.
 2017 Jul 14;13(26):4591-4597.

Anomalous Protein-Protein Interactions in Multivalent Salt Solution.

Pasquier C, Vazdar M, Forsman J, Jungwirth P, Lund M.
J Phys Chem B
. 2017 Apr 13;121(14):3000-3006.


A novel TPR-BEN domain interaction mediates PICH-BEND3 association.
Pitchai GP, Kaulich M, Bizard AH, Mesa P, Yao Q, Sarlos K, Streicher WW, Nigg EA, Montoya G, Hickson ID.
Nucleic Acids Res.
 2017 Nov 2;45(19):11413-11424. 

The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium.

Moroz OV, Blagova E, Lebedev AA, Nørgaard A, Segura DR, Blicher TH, Brask J, Wilson KS. Acta Crystallogr D
Struct Biol.
 2017 Jan 1;73(Pt 1):32-44. 

University of Manchester

Sequence composition predicts immunoglobulin superfamily members that could share the intrinsically disordered properties of antibody CH1 domains.

Hebditch M, Curtis R, Warwicker J.
Sci Rep. 2017 Sep 29;7(1):12404.

Coarse-Grained Modeling of Antibodies from Small-Angle Scattering Profiles.

Corbett D, Hebditch M, Keeling R, Ke P, Ekizoglou S, Sarangapani P, Pathak J, Van Der Walle CF, Uddin S, Baldock C, Avendaño C, Curtis RA.
J Phys Chem B. 2017 Sep 7;121(35):8276-8290.

DprA from Neisseria meningitidis: properties and role in natural competence for transformation.

Hovland E, Beyene GT, Frye SA, Homberset H, Balasingham SV, Gómez-Muñoz M, Derrick JP, Tønjum T, Ambur OH.
. 2017 Jul;163(7):1016-1029.

Structure of the Neisseria Adhesin Complex Protein (ACP) and its role as a novel lysozyme inhibitor.

Humbert MV, Awanye AM, Lian LY, Derrick JP, Christodoulides M.
PLoS Pathog. 2017 Jun 29;13(6):e1006448.

Investigating Liquid-Liquid Phase Separation of a Monoclonal Antibody Using Solution-State NMR Spectroscopy: Effect of Arg·Glu and Arg·HCl.

Kheddo P, Bramham JE, Dearman RJ, Uddin S, van der Walle CF, Golovanov AP.
Mol Pharm.
 2017 Aug 7;14(8):2852-2860.

Protein-Sol: a web tool for predicting protein solubility from sequence.

Hebditch M, Carballo-Amador MA, Charonis S, Curtis R, Warwicker J.
Bioinformatics. 2017 Oct 1;33(19):3098-3100.

The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.

Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP.
Nucleic Acids Res. 
2017 Jul 27;45(13):8064-8078. 

The effect of charge mutations on the stability and aggregation of a human single chain Fv fragment.

Austerberry JI, Dajani R, Panova S, Roberts D, Golovanov AP, Pluen A, van der Walle CF, Uddin S, Warwicker J, Derrick JP, Curtis R.
Eur J Pharm Biopharm.
 2017 Jun;115:18-30.

Evaluation of aggregate and silicone-oil counts in pre-filled siliconized syringes: An orthogonal study characterising the entire subvisible size range.

Shah M, Rattray Z, Day K, Uddin S, Curtis R, van der Walle CF, Pluen A.
Int J Pharm. 2017 Mar 15;519(1-2):58-66.

Influence of Escherichia coli chaperone DnaK on protein immunogenicity.
Ratanji KD, Derrick JP, Kimber I, Thorpe R, Wadhwa M, Dearman RJ.
Immunology. 2017 Mar;150(3):343-355.


Synthesis and biological evaluation of chemokine receptor ligands with 2-benzazepine scaffold.
Thum S, Kokornaczyk AK, Seki T, De Maria M, Ortiz Zacarias NV, de Vries H, Weiss C, Koch M, Schepmann D, Kitamura M, Tschammer N, Heitman LH, Junker A, Wünsch B.
Eur J Med Chem.
 2017 Jul 28;135:401-413. 


Biophysical Analysis of Lipopolysaccharide Formulations for an Understanding of the Low Endotoxin Recovery (LER) Phenomenon.

Correa WBrandenburg KZähringer URavuri KKhan Tvon Wintzingerode F.

Int J Mol Sci. 2017 Dec 16;18(12).

Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires.

Friedensohn S, Khan TA, Reddy ST.
Trends Biotechnol.
 2017 Mar;35(3):203-214.

Approaches to Mitigate the Unwanted Immunogenicity of Therapeutic Proteins during Drug Development.

Salazar-Fontana LI, Desai DD, Khan TA, Pillutla RC, Prior S, Ramakrishnan R, Schneider J, Joseph A.
 2017 Mar;19(2):377-385. 


DTU Chemistry
Oligomerization of a Glucagon-like Peptide1 Analog: Bridging Experiment and Simulations. Tine M. Frederiksen,1 Pernille Sønderby,1 Line A. Ryberg,1 Pernille Harris,1,* Jens T. Bukrinski,2 Anne M. Scharff-Poulsen,2 Maria N. Elf-Lind,1 and Günther H. Peters1,* 1Department of Chemistry, Technical University of Denmark, Kongens Lyngby, Denmark; and 2Novozymes A/S, Bagsværd, Denmark, Biophysical Journal Volume 109, 1202–1213, September 2015.


Analysis of an industrial production suspension of Bacillus lentus subtilisin crystals by powder diffraction: a powerful quality-control Tool, Christian G. Frankaer, Olga V. Moroz, Johan P. Turkenburg, Stein I. Aspmo, Majbritt Thymark, Esben P. Friis, Kenny Stahl, Jens E. Nielsen, Keith S. Wilson and Pernille Harris. Acta Cryst. D70, 1115–1123, 2014.

Max IV Laboratory
BioMAX: The Future Macromolecular Crystallography Beamline at MAX IV. Thunnissen, M. M. G. M., Sondhauss, P., Wallén, E., Theodor, K., Logan, D.T., Labrador, A., Unge, J., Appio, R., Fredslund, F. & Ursby, T., J. Phys.: Conf. Ser. 425 072012, 2013.

Combined SAXS/UV-vis/Raman as a Diagnostic and Structure Resolving Tool in Materials and Life Science Applications, Haas, S.; Plivelic, T. S.; Dicko, C. J.,Phys. Chem. B 118, 2264-2273, 2014.

Exploring genomes for glycosyltransferases, Sara Fasmer Hansen, Emmanuel Bettler, Åsmund Rinnan, Søren B. Engelsen, Christelle Breton, Molecular BioSystems, 6, 1773-1781, DOI: 10.1039/c000238k, 2010.

Recursive weighted partial least squares (rPLS): an efficient variable selection method using PLS, Åsmund Rinnan, Martin Andersson, Carsten Ridder, Søren Balling Engelsen, Journal of Chemometrics, 28 (5), 439-447, DOI: 10.1002/cem.2582, 2014.

Ludvig Maximilian University of Münich
Novel microscale approaches for easy, rapid determination of protein stability in academic and commercial settings, Alexander C.G., Wanner R., Johnson C.M., Breitsprecher D., Winter G., Duhr S., Baaske P., Ferguson N., Biochim Biophys Acta. 2014; 1844(12): 2241–2250. DOI: 10.1016/j.bbapap.2014.09.016.

Temperature-Ramped Studies on the Aggregation, Unfolding, and Interaction of a Therapeutic Monoclonal Antibody, Menzen T., Friess W., J Pharm Sci. 2014; 103(2): 445-455. DOI: 10.1002/jps.23827.

Modulation of the Hydration Water Around Monoclonal Antibodies on Addition of Excipients Detected by Terahertz Time-Domain Spectroscopy. Wallace VP, Ferachou D, Ke P, Day K, Uddin S, Casas-Finet J, Van Der Walle CF, Falconer RJ, Zeitler JA. J Pharm Sci. 2015 Sep 7. doi: 10.1002/jps.24630.

Characterisation of Stress-Induced Aggregate Size Distributions and Morphological Changes of a Bi-Specific Antibody Using Orthogonal Techniques. Hamrang Z, Hussain M, Tingey K, Tracka M, Casas-Finet JR, Uddin S, van der Walle CF, Pluen A. J Pharm Sci. 2015 Aug;104(8):2473-81. doi: 10.1002/jps.24530.

Lund University
Weak Self-Interactions of Globular Proteins Studied by Small-Angle X-ray Scattering and Structure-Based Modeling, Shuji Kaieda, Mikael Lund, Tomás S. Plivelic§, Bertil Halle, J. Phys. Chem. B, 2014, 118:10111.

Dimerization of Terminal Domains in Spiders Silk Proteins Is Controlled by Electrostatic Anisotropy and Modulated by Hydrophobic Patches, Anıl Kurut, Cedric Dicko, and Mikael Lund, ACS Biomater. Sci. Eng., 2015, 1:363.

Bio-reduction of Redox-Sensitive Albumin Conjugates in FcRn-Expressing Cells, Lorine Brülisauer, Gina Valentino, Sakura Morinaga, Kübra Cam, Jens Thostrup Bukrinski, Marc A. Gauthier, and Jean-Christophe Leroux, Angew. Chem. Int. Ed. 2014, 53, 8392-8396

Binding mode of Thioflavin T in insulin amyloid fibrils, Minna Groenninga, Mathias Norrmanc, James M. Flinkb, Marco van de Weerta, Jens T. Bukrinsky, Gerd Schluckebierc, Sven Frokjaer,




Journal of Structural Biology, Volume 159, Issue 3, September 2007, Pages 483–497